Serial Dilution Technique In Microbiology

10/22/2019by admin

Start studying microbiology: serial dilution & disk diffusion. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Sep 28, 2009 A dilution in chemistry is a process that reduces the concentration of a substance in a solution. A serial dilution is the repeated dilution of a solution to amplify the dilution factor quickly. It’s commonly performed in experiments. Oct 08, 2013 A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. The easiest method is to make a series of 1 in 10 dilutions. Serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. From this, a calculation of viable cells in the original suspension can be made, as a colony picked for pure culture. How to Do Serial Dilutions - Performing a Basic Dilution Determine the proper dilution liquid. Prepare several test tubes with 9 mL of dilution liquid. Prepare a test tube with at least 2 mL of your undiluted solution. Perform the first dilution. Perform the second dilution. Sep 09, 2014 This general microbiology practical lecture explains the serial dilution techniques in pour plate method to isolate bacteria. For more information, log on to. Samsung laptop drivers free download.

Serial Dilution Calculations
Serial Dilution Technique

A serial dilution is the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M .. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10^0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10^0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.

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In biology and medicine[edit]

In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results.^[1] Serial dilution is also a cheaper and simpler method for preparing cultures from a single cell than optical tweezers and micromanipulators.^[2]

In homeopathy[edit]

Serial dilution is one of the core foundational practices of homeopathy, with 'succussion', or shaking, occurring between each dilution. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain.^[3]^[4]

Serial Dilution Calculations

References[edit]

^K. R. Aneja. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. 69. ISBN81-224-1494-X
^Booth, C.; et al. (2006). Extremophiles. Methods in microbiology 35. Academic Press. p. 543. ISBN978-0-12-521536-7.
^Weissmann, Gerald (2006). 'Homeopathy: Holmes, Hogwarts, and the Prince of Wales'. The FASEB Journal. 20 (11): 1755–1758. doi:10.1096/fj.06-0901ufm. PMID16940145. Retrieved 2008-02-01.
^Ernst, Edzard (November 2005). 'Is homeopathy a clinically valuable approach?'. Trends in Pharmacological Sciences. 26 (11): 547–548. CiteSeerX10.1.1.385.5505. doi:10.1016/j.tips.2005.09.003. PMID16165225.

Michael L. Bishop, Edward P. Fody, Larry E. Schoeff. Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams & Wilkins, 2004, p. 24. ISBN0-7817-4611-6.

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